Fluorospectroscopic studies of various ganglioside and ganglioside-lecithin dispersions. Steady-state and time-resolved fluorescence measurements with 1,6-diphenyl-1,3,5-hexatriene
- 6 January 1981
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 20 (1) , 162-169
- https://doi.org/10.1021/bi00504a027
Abstract
Molecular motions of 1,6-diphenyl-1,3,5-hexatriene (DPH) in gangliosides (GM3, GM2, GM1, GD1a and GD1b), GA1 glycosphingolipid and dipalmitoyl-sn-glycero-3-phosphorylcholine (DPPC)-ganglioside mixed dispersions were studied by using techniques of steady-state and nanosecond time resolved fluorescence measurements in the temperature range of 20.degree.-50.degree. C. The total fluorescence decay s(t) was approximated to a best-fit curve of double-exponential decays, and 2 fluorescence lifetimes were obtained. The values of the shorter fluorescence lifetime in dispersions composed of a single glycosphingolipid component approached those of the longer one on addition of DPPC. The molecular arrangement or microheterogeneity of the hydrocarbon region surrounding DPH molecules changed depending on the ratio of DPPC to ganglioside molecules and on the temperature. The steady-state anisotropy rs in dispersions composed of a single glycosphingolipid component exhibited smooth changes, not abrupt ones, in the temperature range, in contrast to that in DPPC liposomes. In the various glycosphingolipid dispersions studied, the motion of DPH molecules was the most restricted in the GA1 dispersion. Sialic acid linked to the neutral sugar backbone influenced the hydrophobic region and increased the motion of DPH molecules. In the gangliosides tested, the motion of DPH molecules in the hydrophobic region of GM1 ganglioside was the most restricted. The ultimate and/or penultimate carbohydrate moieties of the neutral sugar backbone of gangliosides and the topographical difference in the locations of the sialic acid linkage influence the integrity of the membranes including the hydrophobic region.Keywords
This publication has 26 references indexed in Scilit:
- The structure and function of glycoprotein hormone receptors: Ganglioside interactions with human chorionic gonadotropinBiochemical and Biophysical Research Communications, 1976
- Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 1. Single component phosphatidylcholine liposomesBiochemistry, 1976
- Interferon action: Role of membrane gangliosidesVirology, 1976
- A calorimetric study of the thermotropic behavior of aqueous dispersions of natural and synthetic sphingomyelinsBiochemistry, 1976
- HIGH-RESOLUTION PREPARATIVE COLUMN CHROMATOGRAPHIC SYSTEM FOR GANGLIOSIDES USING DEAE-SEPHADEX AND A NEW PORUS SILICA, IATROBEADS1976
- The lipid composition of rat-liver plasma membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1968
- GANGLIOSIDES1964
- PHYSICOCHEMICAL PROPERTIES OF OX-BRAIN GANGLIOSIDESBiochemical Journal, 1963
- Tentative Identification of the Tetanus Toxin Receptor in Nervous TissueJournal of General Microbiology, 1959
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957