Evaluation of 24-Second Cyanide-Containing and Cyanide-Free Methods for Whole Blood Hemoglobin on the Technicon H*1™ Analyzer with Normal and Abnormal Blood Samples

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Abstract
Automated methods, with and without cyanide (+CN and −CN), for whole blood hemoglobin (Hb) determination were evaluated on the Technicon H*1™ System. Both automated Hb methods were linear over the range 0–250 g/L (0–25 g/dL) and correlated well with the International Committee for Standardization of Hematology (ICSH) reference method and with the Coulter S+II®. Both methods quantitatively converted whole blood containing up to 100% carboxyhemoglobin in less than 24 seconds to their respective end products. With respect to abnormal samples (sickle cell anemia, multiple myeloma, and hyperlipemia), both H*1 methods gave Hb results that were equivalent to the (postfiltration) ICSH method. For samples with white blood cell (WBC) counts less than 36 × 109/L, the +CN method was equivalent to the (postfiltration) ICSH method, whereas for WBC counts greater than 20 × 109/L, the −CN method showed acceptable recovery of the mean but unacceptable imprecision. For WBC counts of 36–164 × 109/L, the +CN method yielded acceptable Hb recovery with unacceptable imprecision. Hyperlipemia, resulting from addition of Intralipid® directly to the blood samples, caused large errors in both H*1 methods.