DNA restriction-fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat
- 1 August 1986
- journal article
- research article
- Published by Springer Nature in Biochemical Genetics
- Vol. 24 (7-8) , 579-596
- https://doi.org/10.1007/bf00504336
Abstract
Restriction enzyme digests of DNA from nullisomic-tetrasomic and intervarietal chromosome substitution lines of wheat were probed with a high molecular weight (HMW) glutenin cDNA. Three restriction endonucleases were used to investigate restriction-fragment differences among five wheat varieties. The results suggest that the hybridizing fragments contain single gene copies and permit the identification of the subunit encoded by each gene. Restriction-fragment variation associated with previously established allelic differences between varieties was observed. Also, there is a clear relationship between the electrophoretic mobility of a HMW subunit and the length of the central repetitive section of the gene encoding it. These results are discussed with reference to the evolution of the HMW glutenin gene family and the uses of restriction-fragment variation in plant breeding and genetics.This publication has 24 references indexed in Scilit:
- ?-amylase genes of wheat are two multigene families which are differentially expressedPlant Molecular Biology, 1985
- The purification and N-terminal amino acid sequence analysis of the high molecular weight gluten polypeptides of wheatBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984
- Wheat storage proteins: their genetics and their potential for manipulation by plant breedingPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1984
- Characterization of the multigene family coding for HMW glutenin subunits in wheat using cDNA clonesTheoretical and Applied Genetics, 1983
- Regulation of white locus expression: The structure of mutant alleles at the white locus of Drosophila melanogasterCell, 1982
- Structural and genetical studies on the high-molecular-weight subunits of wheat gluteninTheoretical and Applied Genetics, 1982
- Correlations between the inheritance of certain high‐molecular weight subunits of glutenin and bread‐making quality in progenies of six crosses of bread wheatJournal of the Science of Food and Agriculture, 1981
- The Molecular Characterization and Organization of Plant Chromosomal DNA SequencesAnnual Review of Plant Physiology, 1980
- Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endospermTheoretical and Applied Genetics, 1980
- Nullisomic Analysis in Common WheatThe American Naturalist, 1953