In vitro production of viable bacteriophage in a laser plume

Abstract
Bacteriophage .vphi. X174, present at high concentrations in an agar overlay in vitro system, were used as a target for pulsed 2.94 .mu.m (Er:YAG) laser ablation. In this preliminary experiment, the potential for transport of viable viruses in the photoablation plume was investigated for laser energy densities of approximately 3.5 J/cm2 per pulse in exposures of 75-600 laser pulses. Transport of relatively few numbers of viable viruses from the ablation site to the plate assay detector, a distance of approximately 2 cm, was observed.

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