Elephant growth hormone. Isolation and characterization
- 12 January 1987
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 29 (1) , 62-67
- https://doi.org/10.1111/j.1399-3011.1987.tb02230.x
Abstract
Growth hormone has been purified to homogeneity from elephant pituitary glands. It has 191 amino acids with two disulfide bridges and a single tryptophan residue. The somatotropin activity is only 15% when compared with the bovine hormone in the radioreceptor binding assay. From circular dichroism spectra α-helical content of elephant growth hormone is estimated to be 50%. Difference absorption spectra of the hormone suggest the presence of a hydrogen bond between the single Trp and a carboxylate ion.Keywords
This publication has 18 references indexed in Scilit:
- Conformational comparison of human pituitary growth hormone and human chorionic somatomammotropin (human placental lactogen) by second-order absorption spectroscopyArchives of Biochemistry and Biophysics, 1984
- Growth hormone conformation and conformational equilibriumsBiochemistry, 1974
- Aromatic Contributions To Circular Dichroism Spectra Of ProteinCRC Critical Reviews in Biochemistry, 1974
- Human pituitary growth hormone. XXXIII. Comparative studies of the single tryptophan residue in human chorionic somatomammotropin and human pituitary growth hormoneBiochemistry, 1972
- Effects of hydrogen bonding and solvents upon the tryptophanyl 1La absorption band. Studies using 2,3-dimethylindoleBiochemistry, 1972
- Molecular weight and circular dichroism studies of bovine and ovine pituitary growth hormonesBiochemistry, 1972
- Enzymatic iodination of polypeptides with 125I to high specific activityBiochimica et Biophysica Acta (BBA) - Protein Structure, 1971
- PHYSICOCHEMICAL AND STRUCTURAL STUDIES ON BOVINE GROWTH HORMONE*Annals of the New York Academy of Sciences, 1968
- Effect of Light Scattering on Ultraviolet Difference Spectra1Journal of the American Chemical Society, 1960
- Preparation and Properties of Growth Hormone from Human and Monkey Pituitary GlandsScience, 1956