Nature of the antigenic complex recognized by T lymphocytes. I. Analysis with an in vitro primary response to soluble protein antigens.

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Abstract
To analyze the genetic factors involved in the regulation of macrophage-T[thymus-derived]-cell interaction, an in vitro primary response to soluble protein antigen was developed in which nonimmune guinea pig T cells can be sensitized and subsequently challenged in tissue culture with antigen-pulsed macrophages. Antigen[ovalbumin]-specific T-cell activation, as measured by increased DNA synthesis, occurred when syngeneic antigen-pulsed macrophages were used for initial sensitization and secondary challenge. No T-cell activation occurred when allogeneic antigen-pulsed macrophages were used for secondary challenge of cells primed with syngeneic macrophages. When allogeneic antigen-pulsed macrophages were used in primary and secondary cultures it was difficult to assess antigen-specific stimulation due to the substantial mixed leukocyte reaction. When T cells from F1 animals were primed with parental antigen-pulsed macrophages, they responded only to the parental macrophages used for initial sensitization but not to those of the other parent. These results are discussed with respect to T-cell recognition of a complex antigenic determinant which may include I-region gene products.

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