STARCH GEL ELECTROPHORESIS OF COBRA AND RATTLESNAKE VENOMS

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Abstract
The effect of pH on gradient starch gel electrophoresis of the venoms of Crotalus adamanteus and Naja flava has been examined. Sodium acetate buffer, pH 4.1, ionic strength 0.020, appeared most effective for resolution of the former venom, and acetate buffer, pH 4.7, or cacodylate buffer, pH 6.0, for the latter. Two-dimensional starch gel electrophoresis resolved at least 20 zones from the crotaline venom and 11 from the colubrid. Two zones of hemolytic activity were separated from each venom: in C. adamanteus the less cationic zone included possibly two or more acidic proteins; the corresponding zone of N. flava was more basic, more homogeneous, and more active under the conditions of assay.
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