A sulphated glycopeptide in human supragingival calculus extracts
- 1 December 1977
- journal article
- research article
- Published by Springer Nature in Calcified Tissue International
- Vol. 23 (1) , 13-17
- https://doi.org/10.1007/bf02012761
Abstract
A method is described for the isolation and purification of a sulphated glycopeptide from human supragingival calculus. The compound was isolated after using EDTA treatment, 2 M CaCl2 extraction, proteolytic digestion, ethanol precipitation, and finally purified by DEAE cellulose chromatography. It migrated as a single component on cellulose acetate electrophoresis, and chemical and infrared spectral analysis showed the presence of covalently attached sulphate groups. The sulphated glycopeptide was distinguished from being a sulphated glycosaminoglycan.This publication has 27 references indexed in Scilit:
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