VIRTUAL2D: A web‐accessible predictive database for proteomics analysis
- 24 February 2003
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 3 (2) , 129-138
- https://doi.org/10.1002/pmic.200390021
Abstract
The available archive of sequence databases compiled from whole genome projects and budding proteomics efforts have enabled us to develop VIRTUAL2D, an interactive system for the assembly of virtual protein expression maps computed on the basis of theoretical isoelectric focusing point, molecular weight, tissue specificity and relative abundance for any set of proteins currently catalogued. This tool will assist in the preliminary, albeit putative, prediction of the identity and location of unknown and/or low abundance proteins in experimentally derived two-dimensional polyacrylamide gel electrophoresis maps.Keywords
This publication has 20 references indexed in Scilit:
- In-Gel Digestion of Proteins from Long-Term Dried Polyacrylamide Gels: Matrix-Assisted Laser Desorption-Ionization Time of Flight Mass Spectrometry Identification of Proteins and Detection of their Covalent ModificationAnalytical Biochemistry, 2001
- A specialised proteomic database for comparing matrix-assisted laser desorption/ionization-time of flight mass spectrometry data of tryptic peptides with corresponding sequence database segmentsProteomics, 2001
- Mass spectrometric study of different isoforms of the plant toxin saporinJournal of Mass Spectrometry, 2001
- Analysis of glycosyl phosphatidylinositol-anchored proteins by two-dimensional gel electrophoresisElectrophoresis, 2000
- An alternative approach to deal with geometric uncertainties in computer analysis of two-dimensional electrophoresis gelsElectrophoresis, 2000
- The current state of two-dimensional electrophoresis with immobilized pH gradientsElectrophoresis, 2000
- Escherichia coliproteome analysis using the gene‐protein databaseElectrophoresis, 1997
- A comparison of selected mRNA and protein abundances in human liverElectrophoresis, 1997
- Standardized characterization of gene expression in human colorectal epithelium by two‐dimensional electrophoresisElectrophoresis, 1997
- Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteinsElectrophoresis, 1993