HPLC Analysis of Novel Anti-Cancer Agents-Illudins and Their Analogs

Abstract
High performance liquid chromatography combined with liquid extraction and solid-phase extraction were used for determining the serum concentration of new anticancer drugs. Extraction recoveries were evaluated for wide range of concentrations in serum. Adsorption and reversed-phase chromatographic modes were tested for the separation and drugs determination in model mixtures and actual clinical samples. The HPLC assay developed was applied for farmacokinetic Studies of acylfulvene in dog and rats.

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