An Immunological Approach to Gibberellin Purification and Quantification
- 1 July 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 90 (3) , 1148-1155
- https://doi.org/10.1104/pp.90.3.1148
Abstract
Gibberellin (GA) specific, high-affinity monoclonal antibodies have been used to assay the GA content of various plant [Pisum sativum and Marah macrocarpus] tissues and to purify selected GAs by immunoaffinity chromatography. These immunological techniques may not stand alone as a general method of GA analysis. The results of this study indicate, however, that in conjunction with gas chromatography-mass spectrometry for positive GA identification, radioimmunoassay and immunoaffinity chromatography are extremely powerful tools for purifying and quantifying GAs from plant tissues.This publication has 17 references indexed in Scilit:
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