Variable leucine‐rich repeats of tomato disease resistance genes Cf‐2 and Cf‐5 determine specificity
- 26 March 2003
- journal article
- Published by Wiley in Molecular Plant Pathology
- Vol. 4 (3) , 199-202
- https://doi.org/10.1046/j.1364-3703.2003.00162.x
Abstract
SUMMARY The tomato Cf-2 and Cf-5 genes confer race specific resistance to infection by the leaf mould pathogen Cladosporium fulvum. The encoded proteins induce a defence response upon recognition of the fungal Avr2 and Avr5 determinants, respectively. Each resistance protein is comprised largely of leucine-rich repeats (LRRs) and the specificity of recognition is thought to occur through a particular domain. We have investigated this further using domain swaps between Cf-2 and Cf-5. Engineered chimeric genes containing portions of Cf-2 and Cf-5 were expressed and shown to be functional. The results clearly show that the specificity for the particular avirulence determinant is restricted to a region of each gene that encodes a subset of LRRs containing the highest level of intergenic variability. In addition, two non-functional mutants of Cf-5 were characterized and their significance discussed.Keywords
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