H+/e− stoichiometry of mitochondrial cytochrome complexes reconstituted in liposomes Rate‐dependent changes of the stoichiometry in the cytochrome c oxidase vesicles
Open Access
- 19 August 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 288 (1-2) , 179-182
- https://doi.org/10.1016/0014-5793(91)81029-8
Abstract
The H+/e− stoichiometry of protonmotive cytochrome c oxidase, isolated from bovine heart mitochondria and reconstituted in liposomes, has been determined by making use of direct spectrophotometric measurements of the initial rates of e− flow and H+ translocation. It is shown that the ←H+/e− ratio for redox‐linked proton ejection by the oxidase varies from around 0 to a maximum of 1 as a function of the rate of overall electron flow in the complex.Keywords
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