General Method for Suppression of Diagonal Peaks in Heteronuclear-Edited NOESY Spectroscopy
- 29 October 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (46) , 15018-15019
- https://doi.org/10.1021/ja045300l
Abstract
A novel approach is proposed for suppression of diagonal peaks in 15N- and 13C-edited NOESY spectroscopy based on subtracting a spectrum with only diagonal peaks from the conventional NOESY spectrum. This method can be applied to most heteronuclear-edited NOESY experiments. It is far more sensitive than the TROSY-based approach for biomolecules with little TROSY effect, and nearly complete suppression of diagonal peaks can be achieved. The method has been demonstrated on samples of 15N-labeled calmodulin (17 kDa), 13C-labeled DdCAD-1 (24 kDa), and 13C-labeled hemoglobin (65 kDa), showing that cross-peaks very close to diagonals can be assigned reliably based on the difference spectra.Keywords
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