IN VITRO BIOASSAY OF CORTICOTROPIN: MODIFICATION AND STATISTICAL TREATMENT
- 1 May 1955
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 56 (5) , 523-532
- https://doi.org/10.1210/endo-56-5-523
Abstract
The in vitro bioassay of corticotropin, based on secretion of corticoids by isolated rat adrenals, was modified to allow the use of the 4 point assay design and the factorial analysis of Bliss and Marks for the determination of the relative potency, and its error, of an unknown preparation. The average index of precision, [lambda], of 24 assays was 0.15 [plus or minus] 0.007. Of several pituitary preparations only the corti-cotropic preparations are significantly active in this test, indicating that the method is specific for corticotropic activity. The assays of corticotropin by the in vitro method are of the same order as those by the adrenal ascorbic acid depletion method of Sayers, Sayers and Woodbury.Keywords
This publication has 5 references indexed in Scilit:
- CHROMATOGRAPHY OF STEROIDS PRODUCED BY RAT ADRENALS IN VITROCanadian Journal of Biochemistry and Physiology, 1955
- THE MICRO ESTIMATION OF STEROIDS PRODUCED BY RAT ADRENALS IN VITRO1Endocrinology, 1954
- OBSERVATIONS ON IN VITRO ADRENAL STEROID SYNTHESIS IN THE RAT1Endocrinology, 1954
- THE NEED FOR THE PRESENCE OF CALCIUM FOR THE STIMULATIONIN VITROOF RAT ADRENAL GLANDS BY ADRENOCORTICOTROPHIC HORMONEEndocrinology, 1953
- IN VITRO BIOASSAY OF CORTICOTROPHINEndocrinology, 1953