Inverted repeats of Tn5 are transposable elements.
- 1 April 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (8) , 2632-2635
- https://doi.org/10.1073/pnas.79.8.2632
Abstract
Experiments presented here show that each of the 1.5-kilobase inverted repeats of the kanamycin-resistance transposon Tn5 is transposable; we designate them IS50-L (left) and IS50-R (right). By DNA sequence analyses, IS50 is 1533 base pairs (bp) long and generates 9-bp direct repeats of target sequences. The ends of IS50 comprise a hyphenated 8-of-9-bp inverted repeat and are not used with equal efficiency; the outside ends are more active than the inside ends, suggesting that a strong transposase recognition site at the outside ends, suggesting that a strong transposase recognition site at the outside end extends beyond the 8 bp common to both ends.This publication has 23 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.Proceedings of the National Academy of Sciences, 1981
- Structural Analysis of Tn5Published by Cold Spring Harbor Laboratory ,1981
- Transposable elementsCell, 1980
- A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistanceMolecular Genetics and Genomics, 1979
- DNA sequence analysis of the transposon Tn3: Three genes and three sites involved in transposition of Tn3Cell, 1979
- The ends of Tn10 are not IS3.1979
- The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1Nature, 1979
- Tn10 mediated integration of the plasmid R100.1 into the bacterial chromosome: Inverse transpositionMolecular Genetics and Genomics, 1979
- pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs longNucleic Acids Research, 1978