An improved double-tuned and isotope-filtered pulse scheme based on a pulsed field gradient and a wide-band inversion shaped pulse
- 1 December 1996
- journal article
- Published by Springer Nature in Journal of Biomolecular NMR
- Vol. 8 (4) , 492-498
- https://doi.org/10.1007/bf00228150
Abstract
We have developed an improved isotope-filtered pulse scheme in combination with a double-tuned filter, a hyperbolic secant inversion pulse, and a z-filter with a pulsed field gradient. These filtering pulse schemes have been incorporated into several one-, two-, and three-dimensional experiments, which were applied to the 13C/15N uniformly labeled N-terminal SH3 domain of Grb2 complexed with the unlabeled Sos-derived peptide. The proton resonances of the Sos-derived peptide were unambiguously assigned using isotope-filtered DQF-COSY, TOCSY and NOESY spectra. Furthermore, in the isotope-filtered, isotope-edited 3D NOESY spectrum, intermolecular NOEs between the labeled protein and the unlabeled peptide could be identified. Through these applications, we demonstrate the high filtering efficiency of the presented pulse scheme.Keywords
This publication has 13 references indexed in Scilit:
- Fully13C-Refocused Multidimensional13C-Edited Pulse Schemes Using Broadband Shaped Inversion and Refocusing PulsesJournal of Magnetic Resonance, Series B, 1996
- A 13C double-filtered NOESY with strongly reduced artefacts and improved sensitivityJournal of Biomolecular NMR, 1995
- A constant-time 13C−1H HSQC with uniform excitation over the complete 13C chemical shift rangeJournal of Biomolecular NMR, 1995
- Solution structure of the tetrameric minimum transforming domain of p53Nature Structural & Molecular Biology, 1994
- A pulsed field gradient isotope‐filtered 3D 13C HMQC‐NOESY experiment for extracting intermolecular NOE contacts in molecular complexesFEBS Letters, 1994
- The Solution Structures of the trp Repressor-Operator DNA ComplexJournal of Molecular Biology, 1994
- Observation of inter‐subunit nuclear Overhauser effects in a dimeric proteinFEBS Letters, 1993
- Amino acid type determination in the sequential assignment procedure of uniformly 13C/15N-enriched proteinsJournal of Biomolecular NMR, 1993
- Conformation of two non‐immunosuppressive FK506 analogs when bound to FKBP by isotope‐filtered NMRFEBS Letters, 1992
- Solution structure of a calmodulin-target peptide complex by multidimensional NMRScience, 1992