Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.

Abstract
Most of the recently synthesized portion of the chromosome can be obtained after denaturation as small DNA molecules with a sedimentation coefficient of about 10S in a variety of bacterial systems and in 1 bacteriophage system. This supports the mechanism that 2 daughter strands are synthesized in a discontinuous fashion. The secondary structure of the chromosomal region containing these newly synthesized chains may differ from that of ordinary double-stranded DNA.