The ratio of glutamine:glutamate in microalgae: a biomarker for N-status suitable for use at natural cell densities
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Plankton Research
- Vol. 11 (1) , 165-170
- https://doi.org/10.1093/plankt/11.1.165
Abstract
A measure of the N-status of phytoplankton at natural cell densities may be made by determining the ratio of intracellular glutamine:glutamate (GLN:GLU). The intracellular pool of free amino acids from 104.sbd.106 cells is extracted in ultraclean conditions and analysed by reverse phase HPLC. A GLN:GLU ratio of > 0.5 indicates N-replete and < 0.2 N-deplete cells.This publication has 13 references indexed in Scilit:
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