Extraction and HPLC Analysis of Halofuginone in Chicken Serum

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Abstract
Extraction and HPLC methods are described for the analysis of the anticoccidial quinazolinone drug, halofuginone (Hal), in chicken serum. Serum samples were diluted with acetic acid and Hal was adsorbed onto a Bond Elut°C8 column followed by elution and HPLC analysis. Recoveries of Hal from chicken serum spiked with 97 ng/ml Hal after 18 and 48 hours incubation were 97.6 and 96.2%, respectively, and recoveries of a 10 ng/ml spike after 18 and 48 hours incubation were 99.7 and 96.7%, respectively, with a detection limit of 1.5 ng/ml. Hal-HBr fed to chickens at 3 ppm for 10 days resulted in 3.75 ng/ml Hal in the serum.