Construction and Partial Characterization of Recombinant cDNA Clones for Chicken Type I Collagen Messenger RNAs.
- 1 January 1984
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 38b (3) , 237-241
- https://doi.org/10.3891/acta.chem.scand.38b-0237
Abstract
MRNAs for the .alpha.1 and .alpha.2 chains of type I procollagen were partially purified from total embryonic chicken calvaria using gel chromatography on Sepharose 4B and used to construct recombinant cDNA [complementary DNA] clones corresponding to both mRNAs. Restriction site mapping, nucleotide sequencing and hybridization to RNA blots were used to show that clones pCAL1 and pCAL2 contain inserted sequences corresponding to the mRNAs for chicken .alpha.1 and .alpha.2 procollagen chains, respectively.Keywords
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