Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.
- 1 December 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (24) , 7994-7998
- https://doi.org/10.1073/pnas.81.24.7994
Abstract
Poly(A)+ mRNA extracted from embryonic chicken brain and from adult rat brain were fractionated on sucrose density gradients. The fractions were subsequently injected into Xenopus oocytes where the mRNA was translated. The products were processed and incorporated into the oocyte membrane where they formed functional neurotransmitter receptors and voltage-operated channels. Different mRNA fractions induced the incorporation of different transmitter receptors and voltage-operated channels into the oocyte membrane. These experiments provide a useful step towards the understanding of the structure and function of neurotransmitter receptors and channels.This publication has 21 references indexed in Scilit:
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