[3H] Diazepam Displacing Activity in Human Cerebrospinal Fluid
- 5 October 1981
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 37 (4) , 1045-1047
- https://doi.org/10.1111/j.1471-4159.1981.tb04494.x
Abstract
Pooled human cerebrospinal fluid was separated by Sephadex G-50 chromatography. The presence of three peaks, A, B and C, was demonstrated by monitoring absorbance at 254 and 280 nm. All peaks showed [3H]diazepam displacing activity in the membrane receptor test. Peak B was further separated on Bio-Gel P-4. At least two major fractions free of salt and GABA in the molecular weight range of approximately 700-3600 were shown to displace [3H]diazepam in the receptor test. This activity was enhanced by a factor of 3 in the presence of 10 μm-GABA.Keywords
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