Abstract
A simple and rapid method for determination of retinol in whole milk is described. Milk (400 mg) was diluted with a waterethanol mixture (55:9:36 by vol.) and saponified with potassium hydroxide in a culture tube. Retinol was extracted into 10ml heptane-di-isopropyl ether (3:1) of which 100 5 4% and a CV of 3·6%.

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