Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation
- 1 March 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 299 (3) , 235-238
- https://doi.org/10.1016/0014-5793(92)80122-w
Abstract
Prostaglandin F2α (PGF2α) selectively decreases the binding of 125I‐labelled epidermal growth factor ([125I]EGF) to intact Swiss 3T3 cells. Scatchard analysis reveals that PGF2α decreases the number of high‐affinity EGF binding sites without changing the total number of receptors. Prostaglandins E1 (PGE1), E2 (PGE2 or F2β (PGF2β) do not alter the EGF binding to these cells and do not enhance the PGF2α effect. R‐59022 and R‐59949, two diacylglycerol kinase inhibitors, enhance the inhibitory effect of PGF2α, whereas down‐modulation of protein kinase C (PKC) abolishes the effect. These results indicate that PGF2α, decreases EGF binding in Swiss 3T3 cells via PKC activation.Keywords
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