Inhibition of glycolysis by 2‐deoxygalactose in Saccharomyces cerevisiae

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Abstract
The enzymatic steps involved in the inhibition of glycolysis by 2‐deoxygalactose in Saccharomyces cerevisiae have been investigated. Yeast, incubated with 2‐deoxygalactose, accumulates up to 8 mM‐2‐deoxygalactose, 30 mM‐2‐deoxygalactose‐1‐phosphate and 0·25 mM‐UDP‐2‐deoxygalactose and UDP‐2‐dexyglucose. An inverse correlation between 2‐deoxygalactose‐1‐phosphate content and rate of glycolysis has been observed. The intracellular concentration of glycolytic intermediates and related metabolites point to the hexokinase and phosphofructokinase steps as the targets for the inhibition of glycolysis by 2‐deoxygalactose and rule out all other mechanisms that have been proposed to explain this inhibition.