HEMATOPOIETIC STEM-CELLS IN NUDE-MICE TRANSPLANTED WITH "COLONY-STIMULATING-FACTOR-PRODUCING TUMORS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 10  (10) , 874-880
Abstract
When pieces of a human pulmonary carcinoma which was producing colony-stimulating factor (CSF) were transplanted into nude mice, a marked granulocytosis of 106/mm3 was observed in the nude mice 4 wk after the transplantation. To elucidate the control mechanism of hemopoiesis, kinetics of the hemopoietic stem cells of these mice were studied. The number of GM-CFC [granulocyte-macrophage progenitor cell], CFUE [erythroid colony forming units], Meg-CFC [megakaryocyte progenitor cell] and CFUs [pluripotential stem cells] in the whole body increased to 6 times, 8 times, 6 times and 36 times as much, respectively, as those of the control mice. The increase in the number of these progenitors occurred mainly in the spleen. The sera of these mice contained only CSF. The cycling fraction of GM-CFC and CFUs determined by thymidine suicide technique was increased. CSF produced in these mice acted directly or indirectly on CFUs inducing their differentiation into committed stem cells.