Fluorimetric Assay for Serum Albumin Based on Its Enzymatic Activity
- 2 January 1984
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 17 (9) , 857-871
- https://doi.org/10.1080/0003271811681085
Abstract
Serum albumins are known to catalyze the hydrolysis of aryl esters. Both human and bovine albumins are active against Naphthol AS acetate, resulting in a fluorescence excited at 320 nm and monitored at 500 nm. HSA was more active than BSA. At pH 8.0 the reaction is activated by cetyltrimethylammonium bromide. Other esterases in serum require either calcium or a higher pH for activity. The assay is conducted with albumin diluted to about 10−7 M or less, thus dissociating many potentially interfering ligands. Palmitic acid did not interfere. The interference by bilirubin is minimized by using highly dilute albumin. The present method gives results with serum which correlate well with the widely used Bromcresol Green method. Limit of detection for HSA is 14 picomoles.Keywords
This publication has 15 references indexed in Scilit:
- ALKALINE HYDROLYSIS OF A p-NITROPHENYL ESTER CATALYZED BY PROTEIN–SURFACTANT COMPLEXESChemistry Letters, 1982
- Direct demonstration of the highly reactive tyrosine residue of human serum albumin located in fragment 299–585Archives of Biochemistry and Biophysics, 1980
- The reactive tyrosine residue of human serum albumin: Characterization of its reaction with diisopropylfluorophosphateArchives of Biochemistry and Biophysics, 1979
- Effects of drug bindings on esterase activity of human serum albumin. Dissociation constants of the complexes between the protein and drugs such as N-arylanthranilic acids, coumarin derivatives and prostaglandins.CHEMICAL & PHARMACEUTICAL BULLETIN, 1979
- Bovine serum albumin as a catalyst. II. Characterization of the kineticsJournal of the American Chemical Society, 1975
- Bovine serum albumin as a catalyst. Accelerated decomposition of a Meisenheimer complexJournal of the American Chemical Society, 1973
- Albumin standards and the measurement of serum albumin with bromcresol greenClinica Chimica Acta; International Journal of Clinical Chemistry, 1971
- Benzosalicylanilide Ester Substrates of Proteolytic Enzymes. Kinetic and Histochemical Studies. I. Chymotrypsin*Biochemistry, 1964
- Electrophoresis Studies on Blood Plasma Esterases. I. Mammalian Plasmata.Acta Chemica Scandinavica, 1959
- TISSUE DISTRIBUTION OF I131 LABELED HUMAN SERUM ALBUMIN FOLLOWING INTRAVENOUS ADMINISTRATIONJournal of Clinical Investigation, 1955