Simultaneous assessment of inflammation and epidermal proliferation in psoriatic plaques during long-term treatment with the vitamin D3 analogue MC903: modulations and interrelations
- 1 March 1991
- journal article
- Published by Wiley in British Journal of Dermatology
- Vol. 124 (3) , 221-229
- https://doi.org/10.1111/j.1365-2133.1991.tb00565.x
Abstract
The influence of topical application of MC903, an analogue of 1α, 25-dihydroxyvitamin D3, on psoriatic plaques was investigated during a long-term treatment study. The parameters for epidermal growth and for inflammation were assessed on frozen sections using immunohistochemical methods to elucidate their modulations in time and the interrelations between the different cell types involved during treatment with MC903. Biopsies were taken before and after 1, 2, 4 and 12 weeks of treatment. Monoclonal antibodies against the hyperproliferation-associated keratin 16, against cycling nuclei, and against T lymphocytes, B lymphocytes, Langerhans cells and CD14-positive cells were used in combination with a polyclonal antibody against polymorphonuclear leucocyte (PMN)-elastase. The earliest change was a statistically significant decrease in PMN after 1 week of treatment followed by a decline of cycling nuclei after 2 weeks. These changes preceded a decrease of T lymphocytes which occurred after 4 weeks. Keratin 16 content tended to diminish after 4 weeks of treatment. CD14+ cells decreased slightly during the observation period, whereas Langerhans cells tended to increase. No B lymphocytes were found. These results suggest that MC903 influences the number of PMN and epidermal growth rather than the number of T lymphocytes.Keywords
This publication has 26 references indexed in Scilit:
- Treatment of psoriasis by the topical application of the novel cholecalciferol analogue calcipotriol (MC 903)Archives of Dermatology, 1989
- Epidermal Hyperproliferation Assessed by the Monoclonal Antibody Ks8.12 on Frozen SectionsJournal of Investigative Dermatology, 1989
- Improvement of psoriasis by a topical vitamin D3 analogue (MC 903) in a double-blind studyBritish Journal of Dermatology, 1988
- Effects of a novel vitamin D analogue MC 903 on cell proliferation and differentiation in vitro and on calcium metabolism in vivoBiochemical Pharmacology, 1988
- An open study of vitamin D3 treatment in psoriasis vulgarisBritish Journal of Dermatology, 1986
- Successful treatment of psoriasis with topical application of active vitamin D3 analogue, 1α, 24-dihydroxycholecalciferolBritish Journal of Dermatology, 1986
- Effect of 1α,25-Dihydroxyvitamin D3 on the Morphologic and Biochemical Differentiation of Cultured Human Epidermal Keratinocytes Grown in Serum-Free ConditionsJournal of Investigative Dermatology, 1986
- Topical administration of 1,25-dihydroxyvitamin D3 for proriasis: Report of five casesCalcified Tissue International, 1986
- 1,25-Dihydroxyvitamin D 3 : a Novel Immunoregulatory HormoneScience, 1984
- Regulation of Terminal Differentiation of Cultured Mouse Epidermal Cells by lα,25-Dihydroxyvitamin D3*Endocrinology, 1983