Growth of the Eaton Agent of Primary Atypical Pneumonia in Chick Entodermal Tissue Culture.

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Abstract
The Eaton agent of primary atypical pneumonia was carried through two series of 10 and 6 passages, respectively, in chick entodermal cell cultures. Passages were initiated with infected chick embryo lung and were continued using supernatant culture fluid. The theoretical dilution of original inoculum in the terminal passages was 10-11 or greater, indicating growth of the agent in this cell system. Cytopathic effects were not observed and fluorescent antibody staining of infected cultures was negative. The agent was demonstrated in fluids from infected cultures by subinoculation to chick embryos and fluorescent antibody staining of lung sections.