Molecular linkage of the Ly-3 and Ly-2 genes. Requirement of Ly-2 for Ly-3 surface expression.
Open Access
- 15 May 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 140 (10) , 3646-3653
- https://doi.org/10.4049/jimmunol.140.10.3646
Abstract
We have isolated the Ly-3 gene by chromosomal walking from the previously cloned Ly-2 gene. These genes are shown to be 36 kb apart and in the same transcriptional orientation. Transfection of the Ly-3 gene into mouse L cells results in cell surface expression of Ly-3 protein only in the presence of Ly-2 (or its human homolog, CD8), although Ly-2 surface expression is not similarly dependent on Ly-3. cDNA clones encoding Ly-3 have been isolated and sequenced and show little sequence similarity to Ly-2, whereas both Ly-2 and Ly-3 are homologous to Ig variable regions. One cDNA clone encodes a form of Ly-3 lacking a transmembrane region. Although two alleles of Ly-3 have been previously defined serologically, Northern blot analyses of Ly-3 mRNA from a series of inbred mouse strains show unusual polymorphisms in the lengths of Ly-3 mRNA species defining at least three allelic variants of this gene.This publication has 21 references indexed in Scilit:
- The T8 antigen is a multimeric complex of two distinct subunits on human thymocytes but consists of homomultimeric forms on peripheral blood T lymphocytes.Journal of Biological Chemistry, 1983
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity.The Journal of Experimental Medicine, 1981
- IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement.The Journal of Immunology, 1980
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.Proceedings of the National Academy of Sciences, 1979
- Linkage in mice of genes controlling an immunoglobulin kappa-chain marker and the surface alloantigen Ly-3 on T lymphocytesImmunogenetics, 1978
- Evidence for Close Linkage of a Mouse Light Chain Marker with the LY-2,3 LocusThe Journal of Immunology, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977