Transfer of RP4:: Mu to Salmonella typhimurium

Abstract
Restriction-proficient strains of S. typhimurium are ineffective as recipients of normal RP4:: Mucts62 due to the operation of 2 restriction systems (hsdSA and hsdLT) on the Mu moiety of this plasmid. Strains mutant in both these hsd loci are excellent recipients.