ABROGATION OF IL-3 DEPENDENT GROWTH REQUIRES A FUNCTIONAL V-SRC GENE-PRODUCT - EVIDENCE FOR AN AUTOCRINE GROWTH-CYCLE

Google Scholar
  • 1 March 1990
    • journal article
    • research article
    • Vol. 5  (3) , 317-325
Abstract
We have investigated the ability of the v-src ongocene to abrogate growth factor dependent growth in the interleukin-3 dependent myeloid progenitor cell line 32D cl3. Growth factor independent clones were isolated following infection of 32D cl3 cells with murine retroviruses. Containing the v-src oncogene, v-src was demonstrated to be directly responsible for growth factor independence in experiments utilizing temperature-sensitive v-src mutants. The v-src infected cells released a growth factor capable of stimulating the proliferation of normal 32D cl3 cells. Analysis of the mRNA from v-src infected 32D cl3 did not identify the putative autocrine growth factor as one of the currently identified murine or human hematopoietic growth factors.