The development of the intense circular-dichroic signal during granum formation in greening etiolated maize

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Abstract
In greening maize [Zea mays] mesophyll, circular dichroism (c.d.) revealed the early formation of protein-chlorophyll complexes, followed by unorganized chlorophyll. The intense c.d. appeared later still and membrane-membrane contact (stacking), measured from electron micrographs, appeared much earlier. Isolated grana, which still showed stacking, lost 92% of their original intense c.d.; intense c.d. was not therefore simply dependent on stacking.