The DNase I sensitive domain of the chicken Iysozyme gene spans 24 kb

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Abstract
We have determined the DNase I sensitive chromatin domain of the lysozyme gene in the hen oviduct. When nuclei were digested with DNase I, about 14 kb of upstream and 6 kb of downstream sequences in addition to the 4 kb long transcribed region were preferentially degraded. The transcription start site is located near the center of the approximately 24 kb long sensitive domain. At the 3′ boundary there is a rather abrupt transition from the DNase I sensitive to the resistant chromatin configuration whereas at the 5′ border this transition occurs in a gradual fashion over 6–7 kb of DNA. No obvious correlation between the boundaries of the domain and repetitive sequences could be established. DNase I-hypersensitive sites are clustered within the boundaries of the sensitive domain which seems to represent a functional unit of the gene.