ACTION SPECTRA OF PHTHALOCYANINES WITH RESPECT TO PHOTOSENSITIZATION OF CELLS

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Abstract
—: Human carcinoma cells (NHIK 3025 cells) and Chinese hamster cells (V79 cells) were incubated with AIPcS1, AIPcS2and AIPcS4, phthalocyanines with different lipophilicity but with similar photochemical properties when in monomeric solutions. The absorption‐ and fluorescence spectra of the dyes in the cells were recorded as well as their action spectra with respect to sensitizing cells to photoinactivation. These spectra show that under the present conditions AIPcS1is strongly aggregated in both cell lines; AIPcS2is aggregated in V79 cells but much less so in NHIK 3025 cells. A main finding is that the shapes of the action spectra are similar to that of the fluorescence excitation spectra, but not to the absorption spectra, indicating that the photosensitizing effects of the dyes are mainly due to their monomeric fraction in the cells. AIPcS2and AIPcS4localize intracellularly mainly in lysosomes while AIPcS1was found to be more diffusely distributed in cells. As measured per quantum of fluorescence emitted, AIPcS2and AIPcS2are more efficient sensitizers than AIPcS4. The difference in efficiency between AIPcS2and AIPcS4is supposedly due to a different localization pattern on the suborganelle level.

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