Toxicity of 2-deoxygalactose to Saccharomyces cerevisiae cells constitutively synthesizing galactose-metabolizing enzymes.
Open Access
- 1 May 1984
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 4 (5) , 994-996
- https://doi.org/10.1128/mcb.4.5.994
Abstract
Analysis of 400 independent spontaneous mutations conferring 2-deoxygalactose resistance upon cells constitutive for the galactose pathway suggests that toxicity is due to 2-deoxygalactose-1-phosphate. Selection for and against growth on galactose in the same strain is now possible; application to systems with transcriptional or translational gene fusions to galactokinase are discussed.Keywords
This publication has 15 references indexed in Scilit:
- The expression in yeast of the Escherichia coli galK gene on CYCI :: galK fusion plasmidsGene, 1983
- Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1983
- Isolation and preliminary characterization of the GAL4 gene, a positive regulator of transcription in yeast.Proceedings of the National Academy of Sciences, 1982
- Deletion analysis of the Saccharomyces GAL gene cluster: Transcription from three promotersJournal of Molecular Biology, 1981
- The organization and transcription of the galactose gene cluster of SaccharomycesJournal of Molecular Biology, 1981
- Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1981
- Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridizationCell, 1979
- Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiaeMolecular Genetics and Genomics, 1977
- Uninducible mutants in the gal i locus of Saccharomyces cerevisiae.1972
- The enzymatic transformation of uridine diphosphate glucose into a galactose derivativeArchives of Biochemistry and Biophysics, 1951