In vitromodels to evaluate the capacity of different sequestering agents to adsorb aflatoxins

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Abstract
Eight potential aflatoxin-sequestering agents (SAs) were tested for their ability to adsorb aflatoxin B1 (AfB1) and aflatoxin G1 (AfG1) in vitro. They belong to main SA classes: silicate minerals (calcium, magnesium and sodium bentonites, kaolinite, zeolite and clinoptinolite), activated carbon and yeast cell wall-derived. The AfB1 and AfG1 used in present work were extracted from a contaminated corn meal (82.21 mg/kg of AfB1 and 97.20 mg/kg of AfG1). Three single-concentration adsorption tests, consisting of a simply-water (W), a gastro-intestinal simulating monogastric model (MM) and a ruminant model (RM) were used. The methods differed for dilution media, incubation steps and pH condition in which they were conducted. In particular, one step (2h at 39°C) at pH 7 for W; two steps (4h at 39°C) at pH 2 and 7 for MM; and a pre-incubation in rumen fluid (pH 7 for 2h at 39°C) + two steps (4h at 39°C) at pH 2 and 7 for RM, characterized each method. The AfB1:SA ratio (g/g) and dilution factor (ng of incubated...

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