Immunoperoxidase staining in the recognition of Aspergillus infections

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Abstract
The use of the indirect immunoperoxidase technique for labeling Aspergillus spp. in infected human and animal tissues in smears taken from cultures was studied. Specific labeling without serious cross-reactivity with Candida spp. or Zygomycete fungi was demonstrated using antiserum to Aspergillus. It was not possible to distinguish between A. fumigatus and A. flavus with this technique. Pretreatment of sections with sodium azide and normal swine serum was effective in eliminating background staining. The method can be used to produce permanently stained sections and has applications in the recognition of hyphal fragments in tissue from infected patients.

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