Immunohistochemical Studies on the Antigenic Relationship of Some Muscle Tumors to Normal Skeletal Muscle Cells2

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Abstract
Normal skeletal muscle cells, when grown in tissue culture, appeared to shift from the production of myosin to a production of connective-tissue components. That an actual shift of production was taking place and that the observed change in cell population was not entirely due to an outgrowth of one particular type of cell with a decrease growth of another were shown clearly by a paired fluorescent-label technique. Individual cells could be found that contained both connective-tissue components and myosin. This change is of significance in connection with rhabdomyosarcomas in which a similar change would explain the previous observations that some human rhabdomyosarcomas contain myosin while others do not. The latter seem to contain connective-tissue components that are not observed in the rhabdomyosarcomas containing myosin. It would appear that the rhabdomyosarcomas not containing myosin were either not derived from muscle or had lost the ability to produce enough myosin to be seen and were producing enough connective-tissue components to be readily demonstrated.