cdc2 Gene Expression at the G 1 to S Transition in Human T Lymphocytes
- 9 November 1990
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 250 (4982) , 805-808
- https://doi.org/10.1126/science.2237430
Abstract
The product of the cdc2 gene, designated p34cdc2, is a serine-threonine protein kinase that controls entry of eukaryotic cells into mitosis. Freshly isolated human T lymphocytes (G0 phase) were found to have very low amounts of p34cdc2 and cdc2 messenger RNA. Expression of cdc2 increased 18 to 24 hours after exposure of T cells to phytohemagglutinin, coincident with the G1 to S transition. Antisense oligodeoxynucleotides could reduce the increase in cdc2 expression and inhibited DNA synthesis, but had no effect on several early and mid-G1 events, including blastogenesis and expression of interleukin-2 receptors, transferrin receptors, c-myb, and c-myc. Induction of cdc2 required prior induction of c-myb and c-myc. These results suggest that cdc2 induction is part of an orderly sequence of events that occurs at the G1 to S transition in T cells.This publication has 45 references indexed in Scilit:
- The retinoblastoma susceptibility gene product undergoes cell cycle-dependent dephosphorylation and binding to and release from SV40 large TCell, 1990
- Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosisNature, 1989
- In vitro phosphorylation of the tumor suppressor gene RB protein by mitosis-specific histone H1 kinaseBiochemical and Biophysical Research Communications, 1989
- The product of the retinoblastoma susceptibility gene has properties of a cell cycle regulatory elementCell, 1989
- A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cellsCell, 1989
- G 1 /S Transition in Normal Human T-Lymphocytes Requires the Nuclear Protein Encoded by c- mybScience, 1989
- Contingent Genetic Regulatory Events in T Lymphocyte ActivationScience, 1989
- Human cdc2 protein kinase is a major cell-cycle regulated tyrosine kinase substrateNature, 1988
- Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+Nature, 1988
- The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylationCell, 1986