Effect of Human Growth Hormone on Growth of Cells in Tissue Culture1
- 1 January 1962
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 70 (1) , 31-38
- https://doi.org/10.1210/endo-70-1-31
Abstract
The effect of human pituitary growth hormone (HGH) on human cells in tissue culture were studied by determining the changes produced by HGH on the number of nuclei and total protein content of the cells. HGH caused an increase in the rate of nuclear multiplication proportional to the concentration of the hormone and length of incubation time. The relative specificity of the response of the cells to HGH was indicated by the failure of other proteins, including human albumin, bovine somatotropin and human gamma globulin, to produce comparable changes in the rate of growth. The effect of HGH on nuclear multiplication was abolished by the antibody to this hormone.Keywords
This publication has 9 references indexed in Scilit:
- THE EFFECT OF HUMAN GROWTH HORMONE IN MANActa Endocrinologica, 1959
- METABOLIC CHANGES PRODUCED BY HUMAN GROWTH HORMONE (LI) IN A PITUITARY DWARF*Journal of Clinical Endocrinology & Metabolism, 1959
- Effect of Somatotropin on Cells in Tissue CultureScience, 1957
- The Isolation of Gamma Globulin from Blood-Serum by RivanolActa Medica Scandinavica, 1956
- Measurement of Cell Growth in Tissue Culture with a Phenol Reagent (Folin-Ciocalteau)Experimental Biology and Medicine, 1956
- A SIMPLIFIED PROCEDURE FOR THE ISOLATION OF HYPOPHYSEAL GROWTH HORMONEJournal of Biological Chemistry, 1954
- Continuous Subcultivation of Epithelial-like Cells from Normal Human Tissues.Experimental Biology and Medicine, 1954
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.,Experimental Biology and Medicine, 1950