GAS CHROMATOGRAPHY OF BACTERIAL WHOLE CELL METHANOLYSATES.

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Abstract
Strains of “true neisseriae” (Neisseria gonorrhoeae, N. meningitidis, N. cinerea, N. flavescens and the rodshaped N. elongata), “false neisseriae” (N. catarrhalis, N. ovis, and N. caviae) and moraxellae (Moraxella nonliquefaciens, M. bovis, M. lacunata, M. osloensis, M. phenylpyrouvica, M. kingae, and the tentatively named M. urethralis) were analysed for contents of fatty acids. Thirteen fatty acids were identified. Differences in relative amounts of these and additional unidentified components allowed separation, by numerical analysis, of the strains in groups corresponding to genetic affinities. Thus, “true” and “false neisseriae” formed two distinct groups. The fatty acid composition of the rodshaped N. elongata was almost indistinguishable from that of N. flavescens, whereas moraxellae in this respect generally resembled “false neisseriae”. Exceptions were M. kingae and M. urethralis, which both differed considerably from the other organisms studied. The utility of fatty acid analysis in identification of clinical isolates of these bacteria is indicated.

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