SYNTHESIS AND EXPRESSION OF A SYNTHETIC GENE FOR THE ACTIVATED HUMAN C-HA-RAS PROTEIN
- 1 January 1986
- journal article
- research article
- Vol. 77 (1) , 45-51
Abstract
A 576 base-pair DNA encoding human c-Ha-ras protein (p21) with a valine codon at position 12 has been synthesized by ligation of chemically synthesized oligodeoxyribonucleotides. The duplex was inserted into a plasmid with the E. coli tryptophan promoter and was expressed in E. coli efficiently.This publication has 14 references indexed in Scilit:
- Comparative biochemical properties of normal and activated human ras p21 proteinNature, 1984
- Analysis of the transforming potential of the human H-ras gene by random mutagenesis.Proceedings of the National Academy of Sciences, 1984
- Prediction of the three-dimensional structure of the transforming region of the EJ/T24 human bladder oncogene product and its normal cellular homologue.Proceedings of the National Academy of Sciences, 1983
- Spontaneous activation of a human proto-oncogene.Proceedings of the National Academy of Sciences, 1983
- Nucleotide Sequence Analysis of the T24 Human Bladder Carcinoma OncogeneScience, 1983
- Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogeneNature, 1983
- Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologueNature, 1983
- A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogeneNature, 1982
- Mechanism of activation of a human oncogeneNature, 1982
- A gas-liquid solid phase peptide and protein sequenator.Journal of Biological Chemistry, 1981