A Single solution iron-hematoxylin Stain for Intestinal Protozoa
- 1 January 1949
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 24 (1) , 57-60
- https://doi.org/10.3109/10520294909139579
Abstract
A stain for fecal smears is prepared from the following solutions: solution A: 1% hematoxylin in 95% alcohol, prepared by diluting a stock solution of 10% hematoxylin in 95% alcohol; solution B: ferric ammonium sulfuric acid (sp. gr. 1.8), 0.12 ml.; distilled water, 100 ml. Mix equal parts of solution A and solution B; allow to stand overnight, filter and use. For max. length of staining life, store in full, air-tight bottles. To stain fecal smears, fix in Schaudinn''s, pass through iodine alcohol to 50% alcohol, stain for 3 min., wash in running tap water 5-15 min., dehydrate and mount.Keywords
This publication has 5 references indexed in Scilit:
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- Laboratory Diagnosis of Protozoan DiseasesSouthern Medical Journal, 1942
- Some new methods for studying intestinal amoebae and other protozoaParasitology, 1942
- Iron hematoxylins containing ferric and ferrous iron1939
- A Stable, High-Contrast Mordant for Hematoxylin StainingStain Technology, 1936