RAPID CATION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC PROCEDURE FOR THE SEPARATION AND QUANTITATION OF HEMOGLOBINS S, C, AND O-ARAB IN CORD BLOOD-SAMPLES

  • 1 August 1986
    • journal article
    • research article
    • Vol. 108  (2) , 138-141
Abstract
A rapid method for separating and quantitating hemoglobin (Hb) variants in cord blood samples using cation high-performance liquid chromatography (HPLC) is described. The procedure is a modification of a previously published method, and uses a weak cation-exchange Brownlee-3CM column and Bis-Tris-KCN-Na acetate developers. A chromatogram can be completed in 10 minutes. The slow-moving variants, Hbs S, C, and O Arab, can be completely separated from each other and are identified by their elution times relative to Hb A. Hb E elutes as a shoulder on the descending side of Hb A, which is characteristic for this variant in this procedure. Differentiation betweeen the heterozygous, homozygous, and Hb X-.beta.+-thalassemia conditions is easily made.