Leucocyte Migration Inhibitory Activity of Concanavalin‐A‐stimulated Human Lymphocytes

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Abstract
The in vitro effects of heparin, dipyridamole (DIPY) and lysine-acetylsalicylate (LASA) were studied on human peripheral blood leucocyte migration, production/release and the effect of leucocyte migration inhibitory activity (LMIA) from lymphocytes stimulated with concanavalin-A (con-A). The final concentration of each drug was of the same order of magnitude as during clinical treatment. The leucocyte migration under agarose was significantly increased by DIPY at a concentration of 100 .mu.g/ml. The release/production of LMIA was inhibited by DIPY at 1 .mu.g/ml and by LASA at 0.3 .mu.g/ml. Heparin had no influence on LMIA release, even at 10 IU/ml. The LMIA of supernatants from con-A-stimulated lymphocyte cultures was inhibited by DIPY at a concentration of 10 .mu.g/ml, by LASA at 3 .mu.g/ml and by heparin at 10 IU/ml. DIPY and LASA could have a dual role as modifiers of inflammation: the well known role as antiaggregants tending to limit and impede thrombosis, and as antagonists to the lymphocyte-mediated (type IV) immune reaction through a depressive action on lymphokin production/release and activity.