Measurement of sealing resistance of cell-electrode interfaces in neuronal cultures using impedance spectroscopy
- 1 September 1998
- journal article
- Published by Springer Nature in Medical & Biological Engineering & Computing
- Vol. 36 (5) , 630-637
- https://doi.org/10.1007/bf02524436
Abstract
Sealing resistance is highly significant with respect to the electrical neuronelectrode contact because it decreases the stimulation threshold of neurons cultured on a planar micro-electrode array. A method is proposed for measurement of the sealing resistance using impedance spectroscopy. The effect of the sealing resistance on the total impedance spectrum of a cell-electrode interface is modelled for complete coverage of the electrode by the cell. Sensitivity analysis demonstrates that the impedance spectrum is determined by four parameters: two electrode parameters, the sealing resistance and the shunt capacitance between the lead of the electrode and the culture medium. Experimental verification of the model is performed by simultaneous measurement of the impedance spectrum and electrode coverage. A good and unique fit between the simulated and measured impedance spectra was obtained by varying the two electrode parameters and the sealing resistance.Keywords
This publication has 15 references indexed in Scilit:
- Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structuresBiosensors and Bioelectronics, 1997
- Advantages of using microfabricated extracellular electrodes for in vitro neuronal recordingJournal of Neuroscience Research, 1995
- Interfacing cultured neurons to planar substrate microelectrodes: characterization of the neuron-to-microelectrode junctionBioelectrochemistry and Bioenergetics, 1995
- The linear and non-linear electrical properties of the electrode-electrolyte interfaceBiosensors and Bioelectronics, 1995
- Cell manipulation and cultivation under a.c. electric field influence in highly conductive culture mediaBiochimica et Biophysica Acta (BBA) - General Subjects, 1994
- Stimulation of monolayer networks in culture through thin-film indium-tin oxide recording electrodesJournal of Neuroscience Methods, 1993
- Modeling the neuron-microtransducer junction: from extracellular to patch recordingIEEE Transactions on Biomedical Engineering, 1993
- Electrical stimulation and recording from cultured neurons using a planar electrode arrayBioelectrochemistry and Bioenergetics, 1992
- Sealing cultured invertebrate neurons to embedded dish electrodes facilitates long-term stimulation and recordingJournal of Neuroscience Methods, 1989
- Monitoring fibroblast behavior in tissue culture with an applied electric field.Proceedings of the National Academy of Sciences, 1984