An investigation of HPLC post‐column iodination conditions for the enhancement of aflatoxin B1 fluorescence
- 1 October 1984
- journal article
- Published by Taylor & Francis in Food Additives & Contaminants
- Vol. 1 (4) , 325-335
- https://doi.org/10.1080/02652038409385863
Abstract
A systematic investigation was carried out of the conditions employed for the post-column iodination reaction used to enhance fluorescence sensitivity of aflatoxins B1 and G1 in reverse-phase HPLC. The effect of post-column reaction coil tubing dimensions, coil temperature, eluent and reagent flow rates and reagent concentration were examined and the optimum choice of each established. The maximum achievable sensitivity was shown to be 20 pg of B1 injected on column.Keywords
This publication has 3 references indexed in Scilit:
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- Rapid determination of aflatoxin B1 in dutch feeding stuffs by high-performance liquid chromatography and post-column derivatizationJournal of Chromatography A, 1983
- High-performance liquid chromatography of aflatoxins with fluorescence detection.Agricultural and Biological Chemistry, 1978