The effect of ascorbic acid on the metabolism of rat calvarial bone cells in vitro
- 1 September 1989
- journal article
- research article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 7 (5) , 696-701
- https://doi.org/10.1002/jor.1100070510
Abstract
Addition of 50 μg/ml sodium ascorbate to confluent cultures of isolated rat calvarium bone cells resulted in a 21% increase in DNA production, a 50–60% increase in incorporation of [14C]proline into collagenous and noncollagenous proteins, and a 200% increase in alkaline phosphatase activity; under identical conditions, [35S]sulfate incorporation into proteoglycans (glycosaminoglycans) was not affected. These results suggest that ascorbate may be important in maintaining or stimulating the osteogenic phenotype of normal bone cells.Keywords
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